Antimicrobial resistance in fecal E. coli and Salmonella isolates of small poultry flocks in Ontario, Canada

OAHN Poultry Research Project 2018-2019:

Antimicrobial resistance in fecal E.coli and Salmonella isolates of  small poultry flocks in Ontario, Canada

Project Collaborators:

  • Patrick Boerlin (Ontario Veterinary College)
  • Marina Brash (Animal Health Laboratory)
  • Michele Guerin (Ontario Veterinary College)
  • Leonardo Susta (Ontario Veterinary College)
  • Csaba Varga (Ontario Ministry of Agriculture, Food, and Rural Affairs)

A prospective surveillance study of small poultry cases submitted by veterinarians to the Animal Health Laboratory was conducted between October 2015 and September 2017. From each submission a pooled cecal sample was obtained and tested for the presence of fecal E. coli, Salmonella, and Campylobacter. From each positive sample 3 isolates were selected and tested for antimicrobial susceptibility by using a broth microdilution technique.

A total of 433 E. coli isolates (358 chicken, 27 turkey, 24 duck, and 24 game bird), 15 Salmonella isolates (9 chicken, 3 turkey, and 3 duck), and 176 Campylobacter isolates (141 chicken, 21 turkey, 6 duck, and 8 game bird) were recovered.

Moderate (15-39% of isolates) to high (≥40% of isolates) proportion of E. coli isolates were resistant to tetracycline, streptomycin, sulfonamides, and ampicillin. Salmonella isolates were most frequently resistant to streptomycin, tetracycline, and sulfonamides. A high frequency of resistance to tetracycline was observed in the Campylobacter jejuni isolates from chickens (77%) and turkeys (100%), and in the Campylobacter coli isolates from turkeys (50%) and game birds (40%).

The high prevalence of resistance to antimicrobials frequently used to treat poultry bacterial diseases underlines the need of judicious antimicrobial use in small flocks. Our results can be used as a benchmark to measure changes in antimicrobial resistance in enteric bacteria of Ontario small poultry flocks.

To read the full report, click here: Antimicrobial resistance in fecal E. coli and Salmonella isolates of small poultry flocks in Ontario, Canada

Journal Articles

A two-year prospective study of small poultry flocks in Ontario, Canada, part 1: prevalence of viral and bacterial pathogens (Journal of Veterinary Diagnostic Investigation, April 2019)

A two-year prospective study of small poultry flocks in Ontario, Canada, part 2: causes of morbidity and mortality (Journal of Veterinary Diagnostic Investigation, April 2019)

Presentations

Antimicrobial resistance in fecal E.coli and Salmonella isolates of small poultry flocks in Ontario, Canada – Presentation by Michele Guerin from the 2019 CPHAZ meeting.

Posters

Prevalence and Antimicrobial Resistance of Fecal Commensal E. coli and Salmonella Isolates Obtained from Ontario Small Poultry Flocks for presentation by Csaba Varga at AAAP in Washington, DC. August 2019.

 

Causes of Morbidity and Mortality in Small Flocks in Ontario for presentation by Leonardo Susta at AAAP in Washington, D.C., August 2019